Grillberger, Leopold,Reiter, Manfred,Mundt, Wolfgang
申请号：
ES16153131
公开号：
ES2761692T3
申请日:
2011.07.08
申请国别(地区):
ES
年份:
2020
代理人:
摘要：
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS 13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
