A in vitro method for preparing an autologous T cell vaccine for the treatment of multiple sclerosis in a patient made by a process comprising: a) priming in vitro a sample comprising a plurality of mononuclear cells comprising T cells with a combination of fragments of one or more multiple sclerosis associated, antigens b) stimulating the T cells obtained in step a) with antigen presenting cells APC(s) and the combination of fragments of one or more multiple sclerosis associated, antigens, c) propagating the T cells of step b) for vaccination and d) attenuating the propagated cells of c).
