Armando Semedo Mendes,João Ernesto de Carvalho,Marta Cristina Teixeira Duarte,Nelson Eduardo Durán Caballero,Roy Edward Bruns
申请号：
BRPI0100199
公开号：
BRPI0100199B1
申请日:
2001.01.04
申请国别(地区):
BR
年份:
2019
代理人:
摘要：
"Violacein and deoxyviolacein production process optimized by factorial design and response surface analysis by chromobacterium violaceum". It is a procedure for optimizing the production of violacein and deoxyviolacein through experimental designs based on statistical principles. In the initial phase 11 factors were analyzed: carbon source (glucose), yeast extract, methionine, vitamin b12, l-tryptophan, agitation, temperature, ph (phosphate buffer), peptone, inoculum and zinc sulfate concentration. a standard fractional experimental design 2 ^ 15-11 ^ was used. In the second phase a central composite planning involving 5 factors: carbon source (glucose), yeast extract, 1-tryptophan, peptone and zinc sulfate. were chosen based on the result of the previous fractional factorial design. The planning consists of a fractional factorial 2 ^ 5 ^. In the final phase of optimization three factors: carbon source (glucose), yeast extract and l-tryptophan were studied using a central three-factor composite design. the average gross violacein production in this schedule was 0.36 g / l. 50% higher than in the other models studied. The average cell mass for this design was 16.59 g / l, around 2.2 times higher than in other models."processo de produção da violaceína e deoxiviolaceina otimizado por planejamento fatorial e análise de superficie de resposta pela chromobacterium violaceum". trata-se de um procedimento para a otimização da produção de violaceína e deoxiviolaceína através de planejamentos experimentais baseados em princípios estatísticos. na fase inicial 11 fatores foram analisados: fonte de carbono (glicose), extrato de levedura, metionina, vitamina b12, l-triptofano, agitação, temperatura, ph (tampão fosfato), peptona, inóculo e concentração de sulfato de zinco. um planejamento experimental fracionário padrão 2^ 15-11^ foi usado. na segunda fase um planejamento composto central envolvendo 5 fatores: fonte de carbono (glicose), extrato de levedura, l-triptofano, pepton
