The present invention provides a fiber-weaving hygiene item and the fabricating method thereof. The hygiene item comprises a substrate, a fabric-base fiber, and a sensing matter. The fabricating method comprises the following steps of: preparing a substrate, forming a plurality of penetrating holds on the substrate, preparing a fabric-base fiber and interlacing the fabric-base fiber on a first surface and a second surface of the substrate, and disposing a sensing matter on the fabric-base fiber. Wherein the hydrophilicity of the fabric-base fiber is higher than the hydrophilicity of the substrate. The present invention applies the characteristic of the higher hydrophilicity of the fabric-base fiber for luring a testing matter toward the fabric-base fiber. When the testing matter is contacted with the fabric-base fiber, the sensing matter can change its color for bio-examination. Using the present invention, multiple bio-examinations can be done for examining the testing matter.本發明提供一種纖維編織的衛生用具與其製作方法,衛生用具包含一基材、一布基纖維以及一檢測物質。本發明衛生用具製作方法包含以下步驟:準備一基材;於基材上製作複數個穿孔。準備一布基纖維,並將布基纖維經由複數個穿孔交錯穿設於第一表面與第二表面上。將一檢測物質設置於第一表面上之布基纖維。其中布基纖維之親水性高於基材之親水性。本發明利用布基纖維之親水性較基材之親水性為高之特性,將一待測物質導向布基纖維,當待測物質接觸布基纖維時,布基纖維之檢測物質會變色以達成生物檢測之效。藉由本發明,可對待測物質進行多類型的生物檢測。1‧‧‧衛生用具10‧‧‧檢測層11‧‧‧基材111‧‧‧第一表面112‧‧‧第二表面12‧‧‧穿孔13‧‧‧布基纖維14‧‧‧檢測物質15‧‧‧混合漿體16‧‧‧防水密封膠20‧‧‧膠膜30‧‧‧吸水層40‧‧‧疏水層50‧‧‧待測物質M‧‧‧膠體