James J. Cali,Dieter Klaubert,William Daily,Samuel Kin Sang Ho,Susan Frackman,Erika Hawkins,Keith V. Wood
申请号：
US15408662
公开号：
US10408819B2
申请日:
2017.01.18
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible lucifetrase inhibitors.
