Mark A. Emalfarb,Peter J. Punt,Cornelia Van Zeijl,Cornelius Van Den Hondel,Jan Verdoes,Richard P. Burlingame
申请号：
US12518595
公开号：
US20120030839A1
申请日:
2007.12.10
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.
