The invention concerns a method of three-dimensional image reconstruction from a set of acquired two-dimensional images (11). A subsampling (12) is taken of the set of acquired two-dimensional images so as to reduce their resolution. A first analytical algorithm of three-dimensional image reconstruction from low-resolution (LR) acquired two-dimensional images (13) is applied (14) in order to obtain a low-resolution three-dimensional image. A reconstruction support (18) is determined (17) by selecting in the low resolution three-dimensional image (16) a set of particular voxels. One then applies (21) a second analytical algorithm of three-dimensional image reconstruction (22) from acquired two-dimensional images (11) and considering only the voxels of the reconstruction support in order to obtain the high-resolution (HR) three-dimensional image.
