The invention provides a method for detecting cardiovascular disease, which comprises the following steps: providing a biological sensing transistor which includes a base and a response electrode. The base has a source, a drain and a gate surface disposed therebetween. The response electrode is spaced apart from the gate surface of the base, and has an electrode body and a receptor immobilized thereon for binding with a cardiovascular disease biomarkers, and applying a pulse voltage that has tunable pulse width and pulse height to the response electrode, causing in a voltage difference between the response electrode and the gate surface, and measuring a detection current which generates from the base in pulse width as to obtain the first sensing indicator.一種心血管疾病的檢測方法,包含以下步驟:準備一包括一基體,及一與該基體間隔設置的反應電極的生物感測電晶體,該基體具有一源極、一與該源極隔設置的汲極,及一位於該源極與該汲極之間的閘極端,該反應電極具有一電極本體,及設置於該電極本體表面且與該電極本體相互鍵結的受體,及將一能與該受體產生反應的心血管疾病生物標記液結合於該受體上,並施加一可調變脈波寬度與高度的脈波電壓於該反應電極,以令該反應電極與該閘極端之間產生一壓差,並於該脈波寬度內量測運算自該基體產生的檢測電流,得到一第一感測指標。