A non-invasive blood glucose measuring device and a measuring method using the same are provided. The non-invasive blood glucose measuring device includes a signal emitting module, a delay component, a wave mixing component and a signal processor. The signal emitting module is configured to emit a detection signal. The delay component is configured to delay the detection signal to become a delay signal. The wave mixing component is configured to mix a return signal and the delay signal to form a mixed signal, wherein the return signal is the detection signal reflected from an object. The signal processor is configured to obtain a blood glucose level of the object according to the mixed signal.