PROCÉDÉ DE PRODUCTION D'UN PHOSPHOLIPIDE À PURETÉ ÉLEVÉE ET D'UNE SPHINGOMYÉLINE À PURETÉ ÉLEVÉE ET GLYCÉROPHOSPHOLIPIDE DU TYPE PLASMALOGÈNE OBTENU AU MOYEN DUDIT PROCÉDÉ
UMEDA JIMUSHO LTD.;MARUDAI FOOD CO., LTD.;BOOCS MEDICAL CORPORATION
发明人:
申请号：
EP09766757.0
公开号：
EP2308954B1
申请日:
2009.06.19
申请国别(地区):
EP
年份:
2012
代理人:
摘要：
This invention provides a process for producing a high-purity sphingomyelin and a high-purity plasmalogen-form glycerophospholipid from a biological material by simple procedures at high yields. The process comprises the steps of: (A) subjecting dried total lipids extracted from a biological material to extraction treatment with a specific mixture solution to separate an insoluble portion composed mainly of sphingomyelin and a soluble portion； (B) subjecting the insoluble portion, obtained in said Step (A), to washing treatment with a specific mixture solution to obtain crude sphingomyelin； (C) subjecting the soluble portion, obtained in said Step (A), to washing treatment with a water-soluble ketone solvent to obtain crude plasmalogen-form glycerophospholipid； (D) causing an enzyme to act on the crude sphingomyelin, obtained in said Step (B), to obtain sphingomyelin having a purity of 90% or more； and (E) causing an enzyme to act on the crude plasmalogen-form glycerophospholipid, obtained in said Step (C), to obtain plasmalogen-form glycerophospholipid having a purity of 40% or more.
