A new and distinct Aloe aristata x Haworthia limifolia hybrid plant named ‘;AMIAL1601’;, which is characterized by long, broad and thick succulent foliage, foliage arranged in multiple whorls which, together, create a tall rosette, and dark green foliage with very light green orbicular protuberances arranged in irregular, wavy transverse rows across the upper and lower leaf surfaces. The new variety has shown to be uniform and stable in the resulting generations from asexual propagation.