IMPERIAL COLLEGE INNOVATIONS LIMITED;MICROSCIENCE LIMITED
发明人:
HOLDEN, DAVID WILLIAM,SHEA, JACQUELINE ELIZABETH,HENSEL, MICHAEL
申请号：
CA2623339
公开号：
CA2623339C
申请日:
1995.12.11
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of: (1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism； (2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant； (3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment； (4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms； (5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marker sequence of each individual mutant stored as in step (2)； and (6) selecting an individual mutant which does not contain any of the marker sequences as isolated in step (4).
