IMPROVED PROCESS FOR EXPRESSION, PURIFICATION AND ENHANCED RECOVERY OF MYCOBACTERIAL RECOMBINANT PROTEINS

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专利权人:

发明人:

TYAGI ANIL K,CHAUDHARRY VIJAY K,GUPTA AMITA,KULSHRESTHA ABHISHEK

申请号：

IN2077/DEL/2004

公开号：

IN263766B

申请日:

2004.10.25

申请国别(地区):

IN

年份:

2014

代理人:

摘要：

The present invention relates to cloning, expression in E coli and purification of mycobacterial antigens. The invention also in particular relates to expression and purification of two important mycobacterial proteins with serodiagnostic potential, namely, 38-kDa and Mtb81 antigens, in monomeric form which involves using a T7 promoter based expression vector under conditions of regulated and slow expression followed by 3-step column chromatography procedure to obtain highly purified proteins with enhanced yields of two proteins. The invention further relates to use of purified proteins in detecting antibodies in sera of tuberculosis patients .

来源网站：

中国工程科技知识中心

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http://www.ckcest.cn/home/