A formulation for prevention of hair loss includes a first agent. The first agent has a pH range from 7.5 to 9.0 and includes a thermostable enzyme, wherein the thermostable enzyme is active in a temperature range greater than 32℃.一種預防落髮的配方,包含第一劑。第一劑具有7.5至9.0的pH值範圍且含有熱穩定酵素,其中該熱穩定酵素具有大於攝氏32度的一活性溫度範圍。