МИД Томас (US),НАРВА Кеннет (US),ХЕЙ Тимоти (US),ЛАРРИНУА Игнасио (US),ВУСЛИ Аарон Т. (US),БЕРТОН Стефани Л. (US)
申请号：
RU2012130019/10
公开号：
RU2012130019A
申请日:
2010.12.16
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A variant of the Cry1Ca protein that has insecticidal activity in which all or parts of the N-terminal alpha helices 1, 2A and / or 2B of the corresponding wild-type Cry1Ca are deleted. The protein variant of claim 1, wherein the deletions remove the entire α-helix 1 and all or part of the α-helix 2 in domain I, wherein said protein contains α-helices 3 through 7.3. A protein variant according to claim 1, wherein said deletions increase the insecticidal activity of the insecticidal protein DIG-109, where said deletions begin before the start of the α-helix 2A and end after the end of the α-helix 2B, but do not continue to the α-helix 3.4. A protein variant according to claim 1, wherein said deletions increase the insecticidal activity of the insecticidal protein DIG-152, where said deletions begin before the start of the α-helix 2A and end after the end of the α-helix 2B, but do not continue to the α-helix 3.5. The protein variant of claim 1, wherein the N-terminal deletions begin with at least one destabilizing amino acid, and said protein contains an added codon that defines the glycine amino acid between the translation-initiating methionine and the destabilizing amino acid. A protein variant according to claim 1, wherein said protein lacks the C-terminal protoxin sequence. Modified Cry1Ca protein containing an integrated protease cleavage site in the spacer region between the α-helix 2B and the α-helix 3.8. The modified protein according to claim 7, wherein said protease cleavage site is inserted within amino acids 85 to 90 of the central toxin protein Cry1Ca SEQ ID NO: 1.9. The protein of Claim 1 or Claim 7, wherein said protein has increased activity against insects. The protein of claim 9, wherein said insect is selected from the group consisting of grass scoops and �1. Вариант белка Cry1Ca, который обладает инсектицидной активностью, в котором все или части из N-концевых альфа-спиралей 1, 2A и/или 2B соответствующего Cry1Ca дикого типа удалены.2. Вариант
