Systems for non-contact imaging measurement of blood oxygen saturation and perfusion in a sample are provided including a control unit configured to facilitate acquisition of data from a sample； a data acquisition module coupled to the control unit, the data acquisition module configured to illuminate a field of view (FOV) of the sample using a plurality of wavelengths to provide a plurality of images corresponding to each of the plurality of wavelengths responsive to control signals from the control unit； and an image processing module configured calculate image saturation parameters and reflectance for each of the plurality of images having a unique acquisition time and unique wavelength and extracting blood volume and oxygen saturation data in the FOV using the calculated image saturation parameters and reflectance for each of the plurality of images having a unique acquisition time and unique wavelength.
