An eye state detection system includes an image processor and a deep learning processor. After the image processor receives an image to be detected, the image processor identifies an eye region from the image to be detected according to a plurality of facial feature points, the image processor performs image registration on the eye region to generate a normalized eye image to be detected, the deep learning processor extracts a plurality of eye features from the normalized eye image to be detected according to a deep learning model, and the deep learning processor outputs an eye state in the eye region according to the plurality of eye features and a plurality of training samples in the deep learning model.