A rectal mucosal administration preparation of Pulsatilla chinensis (Bge.) Regel saponin B4, comprising Pulsatilla chinensis (Bge.) Regel saponin B4 and a pharmaceutically acceptable substrate； the rectal mucosal administration preparation is one or two selected from among a rectal gel and a rectal suppository. Specifically, provided are a rectal gel of Pulsatilla chinensis (Bge.) Regel saponin B4, a rectal suppository of Pulsatilla chinensis (Bge.) Regel saponin B4 and preparation methods therefor. Compared with oral administration, the rectal mucosal administration preparation of Pulsatilla chinensis (Bge.) Regel saponin B4 has a lower effective dose, and has a better medicinal effect at the same dose.
