BP CORPORATION NORTH AMERICA INC.;SYNGENTA PARTICIPATIONS AG
发明人:
Brian Steer,Walter Niles Callen,Shaun Healey,Derrick Pulliam
申请号:
US14019460
公开号:
US20140199705A1
申请日:
2013.09.05
申请国别(地区):
US
年份:
2014
代理人:
摘要:
The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.