Hampson, Brian,Goltry, Kristin,Smith, Douglas M.,Rowley, Jonathan A.,Venturi, Naia
申请号：
AU2013206071
公开号：
AU2013206071B2
申请日:
2013.05.29
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
#$%^&*AU2013206071B220160519.pdf#####ABSTRACT The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash 5 step allows for the production of TRC populations with greater tissue repair and antiinflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within an aseptically closed cell culture 0 chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use. In such embodiments, the final composition is a substantially purified and concentrated cell mixture suspended in a physiologic solution suitable for immediate use in humans without further washing, volume reduction, or processing. Embodiments are also 5 applicable to harvesting (and/or washing) particles within a liquid or solution within a chamber.
