The invention belongs to biotechnology field； specifically the design, preparation methods and applications of variants of annexin V are disclosed. A variant of annexin V is the protein with amino acid residue sequence of sequence 1 in the SEQUENCE LISTING, characterized in that it is a protein derived from the sequence 1 by one or several amino acid residues of the sequence 1 being substituted, deleted or added and which has the same activity as the amino acid residue sequence of the sequence 1. The variant protein of annexin V prepared from the preparation methods of the variant of annexin V has high purity, high productivity, high labeling efficiency and stability, without effect to the biological function of the variant of annexin V, is applicable to industrial production, and lays the foundation for further research of the variant of annexin V and its applications as monitoring reagents of cell apoptosis, monitoring drugs of disease etc.
