PETER R BEETHAM,PATRICIA L AVISSAR,KEITH A WALKER,RICHARD A METZ
申请号:
PT09172695
公开号:
PT2135504E
申请日:
2000.10.10
申请国别(地区):
PT
年份:
2013
代理人:
摘要:
The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycinc family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic Oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvyl-shikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene.