Provided herein are tandem Fcs and tandem Fc antibodies (“TFcAs”), e.g., tandem Fc bispecific antibodies (“TFcBAs”), which comprise one or at least two binding sites that specifically bind to one or more cell surface receptors. The binding sites are connected through a TFc, which TFc comprises a first Fc region and a second Fc region, wherein the first and the second Fc regions are linked through a TFc linker to form a contiguous polypeptide and dimerize to form an Fc dimer. Exemplary TFcBAs bind to the cell surface receptors c-Met and EpCam and inhibit signal trans duction through the cell surface receptor(s) for which the binding sites of the TFcBA are specific.本文提供串聯Fc及串聯Fc抗體(「TFcA」),例如串聯Fc雙特異性抗體(「TFcBA」),其包含一個或至少兩個特異性結合於一或多種細胞表面受體之結合位點。該等結合位點經由TFc連接,該TFc包含第一Fc區及第二Fc區,其中該第一Fc區與該第二Fc區經由TFc連接子連接形成連續多肽,且二聚化形成Fc二聚體。例示性TFcBA結合於細胞表面受體c-Met及EpCam且抑制經由對該TFcBA之結合位點具特異性之細胞表面受體進行之信號轉導。
