An object of the present invention is to provide, for example, an agent for promoting migration of pluripotent stem cells containing an extract from inflamed tissues inoculated with vaccinia virus. The present invention has proven that an extract from inflamed tissues inoculated with vaccinia virus promotes migration of Muse cells. Thus, the present invention provides, for example, an agent for promoting migration of pluripotent stem cells for various diseases to which migration of pluripotent stem cells may have an advantageous effect.