The present invention relates to a subculture method of Lobella sokamensis , and the subculture method of the Pouring pattern earthenware of the present invention has a better survival rate of adult tortoise pattern in soil than the conventional culture method activated carbon-gypsum mixed medium. It is maintained, and it is confirmed that the reproduction to the next generation is well performed, and thus there is an effect of increasing the ease of application of the kidnapping pattern earthenware as a biological indicator. In addition, the subculture method of the present invention contributes to effective management of biological species, and thus is highly applicable in the field of soil ecological toxicity.본 발명은 꼬마혹무늬톡토기(Lobella sokamensis) 계대배양 방법에 관한 것으로, 본 발명의 꼬마혹무늬톡토기 계대배양 방법은 기존의 배양 방법인 활성탄-석고 혼합배지보다 토양에서 꼬마혹무늬톡토기 성충 생존율이 양호하게 유지되며 다음세대로의 번식이 잘 되는 것을 확인하였으므로, 생물지표로써 꼬마혹무늬톡토기의 적용 용이성이 증대시킬 수 있는 효과가 있다. 또한, 본 발명의 계대배양 방법은 효과적인 생물종 관리에 기여하여 토양 생태독성분야에서 활용가능성이 높다.
