STUIVER MAARTEN HENDRIK,PONSTEIN ANNE SILENE,OHL STEPHAN ANDREAS,GODDIJN OSCAR JOHANNA MARIA,SIMONS LAMBERTUS HENRICUS,DEKKER BERNARDUS MARTINUS MARIA,HOEKSTRA SIETSKE,TIGELAAR HENDRIK,ELZINGA NICOLAS
申请号：
US19990469812
公开号：
US7029908(B1)
申请日:
1999.12.22
申请国别(地区):
美国
年份:
2006
代理人:
摘要：
The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.
