The present invention relates to a large quantity production of Rg3 (r form) which is one among water-insoluble saponin, from the leaves and roots of ginseng naturally grown domestically. More particularly, the large quantity production of Rg3 comprises: a ginseng material pre-treatment step of washing the roots or leaves of ginseng, naturally drying the same, and pulverizing the resultant product a ginseng saponin extraction and conversion step of converting water-soluble saponin Rg3(s) into water-insoluble saponin Rg3(r) by obtaining an extract by adding alcohol to the pulverized material, removing chlorophyll, and passing the resultant product through a high temperature and high pressure treatment, frying treatment, and enzyme treatment and a step of recovering and purifying water-insoluble saponin Rg3(r) having high purity and white crystals by obtaining a ginseng extract precipitated by diluting a ginseng extract with water, obtaining a water-insoluble saponin fraction by using the resultant product and MPLC, splitting water-insoluble saponin Rg3(r) by using HPLC, and by freeze-drying the resultant product. The present invention relates to a technique to produce a large quantity of water-insoluble Rg3(r) as a material which can replace water-soluble Rg3(s) derived from ginseng, which is recently used as a raw material for an anti-cancer medicine. Particularly, the present invention relates to a process for preparing water-insoluble Rg3(r) by using the root hair of ginseng, which has relatively cheap price by having low marketability, and leaves of ginseng after harvest, which does not have any value.COPYRIGHT KIPO 2017본 발명은 국내에 자생하는 인삼의 잎과 뿌리로부터 비수용성 사포닌 중의 하나인 Rg3(r form)의 대량생산에 관한 것이다. 보다 상세하게는 인삼의 뿌리나 잎을 수세하고 자연건조한 후 분말화하는 인삼 재료의 전처리 단계와, 분말화된 재료에 주정을 첨가하여 추출물을 얻고 엽록소를 제거한 후, 고온 고압처리, 유탕처리 및 효소처리하여 수용성 사포닌 Rg3(s)을 비수용성 사포닌 Rg3(r)로 전환시키는 인삼 사포닌의 추출 및 전환 단계와, 인삼추출물을 물로 희석하여 침전된 비수용성 사포닌 Rg3(r) 얻고, 이를 MPLC를 이용하여 비수용성 사포닌 분획을 얻은 다음, HPLC를 이용하여 비수용성 사포닌 Rg3(r)를 분
