I to provide a process for the preparation of sugar solution composed of heat-resistant acidophilic bacteria growth factor of propagating heat-resistant acidophilic bacteria is a major cause of bacterial spoilage of food is reduced or removed. To prepare a sugar solution through the following steps: Step of adding an acid to the (a) raw sugar solution, Step of recovering as the target sugar solution (c) and process filtrate and filtered through a filter membrane charged sugar solution prepared (b) in the step (a).飲食品の変敗の主な原因菌である耐熱性好酸性菌を増殖させる耐熱性好酸性菌増殖ファクターが除去または低減されてなる糖液の調製方法を提供する。下記の工程を経て糖液を調製する:(a)原料糖液に酸を加える工程、(b)前記(a)工程で調製した糖液を荷電濾過膜で濾過する工程、及び(c)濾液を上記目的糖液として回収する工程。