PROBLEM TO BE SOLVED: To provide a manufacturing method of an oil-in-water emulsion including a flavoring agent, allowing the aroma and taste of flavoring agent to be well developed, having mellow aroma and taste, eliminating unpleasant aroma and taste while having excellent flavor, with creamy taste derived from the fat in the oil-in-water emulsion.SOLUTION: The manufacturing method of an oil-in-water emulsion made from fat, a flavoring agent, an emulsifier, and water includes separately using the flavoring agent and the emulsifier in preparation of the oil-in-water emulsion. An oil-in-water emulsion (A) is prepared in advance from fat, an emulsifier, and water. An oil-in-water emulsion (B) is then prepared from the oil-in-water emulsion (A) using a flavoring agent, so as to be subjected to disinfection or sterilization treatment. The agitation energy in preparation of the oil-in-water emulsion (A) is 0.05 J/L or more. The oil-in-water emulsion (B) is thus manufactured.