PROBLEM TO BE SOLVED: To provide a method for producing a carotenoid having 55 or 60 carbon atoms.SOLUTION: Provided is a mutant diapophytoene synthase gene in which phenylalanine at amino acid position 233 is substituted with serine. The method obtains a carotenoid having 55 or 60 carbon atoms from a culture product cultured in a culture medium from cells transformed with the mutant diapophytoene synthase gene and mutant farnesyl diphosphate synthase gene or hexaprenyl diphosphate synthase gene.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2017,JPO&INPIT【課題】炭素数55又は60のカロテノイドを製造する方法の提供。【解決手段】アミノ酸配列233番目のフェニルアラニンをセリンへ置換した変異型ジアポフィトエン合成酵素遺伝子。変異型ジアポフィトエン合成酵素遺伝子及び変異型ファルネシル二リン酸合成酵素遺伝子、又はヘキサプレニル二リン酸合成酵素遺伝子により形質転換された細胞を培地で培養し、培養物から炭素数55又は60のカロテノイドを得る方法。【選択図】図1