To provide an image pickup unit that can prevent axis deviation of a movable lens barrel and obtain high-quality picked-up image, a stopper member 58 is positioned and fixed in a state in which the stopper member 58 is abutted against an outer circumferential surface of a rear group lens barrel 57, movement of a movable lens barrel 65 to a retraction side along a photographing optical axis O is restricted by contact of a stopper section 58b of the stopper member 58 and an operation rod 65a, and a movable lens 66 is held in a position for realizing a second focal length.