The present invention relates to a method for separating one or more oligosaccharide compound(s) from a preliminary dairy source, the method comprises the steps of: (i) Providing the preliminary dairy source； (ii) Contacting the preliminary dairy source with a chromatographic support allowing one or more oligosaccharide compound(s) present in the preliminary dairy source to be retained by the chromatographic support； (iii) Obtaining a unretained, flow through fraction from the chromatographic support comprising a secondary dairy source； (iv) Optionally washing the chromatographic support； and (v) Subjecting the chromatographic support to at least one elution buffer obtaining one or more oligosaccharide compound(s) from the chromatographic support； wherein the chromatographic support comprises one or more adsorbent capable of specifically binding the one or more oligosaccharide compound(s) from the preliminary dairy source.
