Randall S. Prather,Rongfeng Li,Clifton N. Murphy,Lee Spate
申请号：
US13061222
公开号：
US08852078B2
申请日:
2009.08.28
申请国别(地区):
US
年份:
2014
代理人:
摘要：
The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the NP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.
