VILKIN JAKOV FIMOVICH,Вилькин Яков Фимович (RU),MARKIN SERGEJ SERGEEVICH,Маркин Сергей Сергеевич (RU),SEMENOV ANDREJ MIKHAJLOVICH,Семенов Андрей Михайлович (RU)
申请号：
RU2011111094/10
公开号：
RU0002448158C1
申请日:
2011.03.24
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
FIELD: medicine.SUBSTANCE: supernatant containing a recombinant protein with an amino acid sequence of staphylokinase (RPSF) is defrozen. The RPSF is recovered and chromatographically purified on CM-sepharose. The RPSF is chromatographically purified on DEAE-sepharose. Then the RPSF is transferred into 0.3% NaCl solution. It is followed by sterilisation, packaging, lyophilisation and marking of the substance. 20 litres of water for injections are introduced in a stirred reactor 160 l, then 150 g of the substance and 10 l of water for injections are added the stirrer is actuated (stirring rate is 80 rpm) the stirring procedure is carried out for 15 min thereafter the stirrer is stopped the substance is sampled the pH value of the prepared solution is recorded pH is 5.5 to 7.5. It is followed by sterilisation filtration of the solution, filling and pre-corking of bottles. Then lyophilisation and final corking are performed. What is offered is the application of the preparation Fortelysin for treating myocardial infraction by the intravenous introduction in dose 15 mg (2235000 IU) with the content of a bottle 5 mg (745000 IU) before the introduction of the preparation is dissolved in 0.9% sodium chloride 5 ml the preparation is introduced in two boluses 10 mg (1490000 IU) simultaneously and in 30 minutes 5 mg (745000 IU) or at first 10 mg (1490000 IU) in boluses, and the rest 5 mg (745000 IU) in intravenous infusions in 0.9% sodium chloride 50 ml for 30 min.EFFECT: invention extends the range of drug preparations with fibrinolytic properties.3 clИзобретение относится к биотехнологии и фармакологии, в частности к способу получения препарата Фортелизин, обладающего фибринолитическими свойствами, и его применению для лечения инфаркта миокарда. Размораживают супернатант, содержащий рекомбинантный белок с аминокислотной последовательностью стафилокиназы (РБСФ). Выделяют и хроматографически очищают РБСФ на СМ-сефарозе. Производят хроматографическую очистку РБСФ на DEAE-сефар
