NUCLEIC ACID, EXPRESSION CASSETTE, VECTOR, CLONING VEHICLE, MOTRANSGENIC MICROORGANISM, POLYPEPTIDE, SIGNAL PEPTIDE, COMPOSITION, AND METHODS TO PRODUCE A RECOMBINANT CARIAN CARD A NON-CARBONIZED LIP FOR A NERCARIOUS CARBON TO FORM A CARBON-CARBON CONNECTION, TO MAKE A FOOD, FEED OR DRINK, AND TO CONVERT A BIOMASS ANY LIGNOCELLULOSTIC MATERIAL INTO A FUEL
DAVID P. WEINER,ELLEN BURKE,LISHAN ZHAO,PAULA M. HICKS,PETER LUGINBUHL,TOBY RICHARDSON
申请号：
BR122017003017
公开号：
BR122017003017B1
申请日:
2007.03.07
申请国别(地区):
BR
年份:
2018
代理人:
摘要：
This invention relates to polypeptides with aldolase activity, including pyruvate activity such as, without limitation, hmg and / or khg aldolase activity, polynucleotides encoding such polypeptides, and methods for producing and using such polynucleotides and polypeptides. In some embodiments, the present invention relates to aldolase activity polypeptides, including pyruvate activity such as, without limitation, hmg and / or khg aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding such enzymes, and to production and use of these polynucleotides and polypeptides. Polypeptides according to the invention may be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways which are useful in the production of r-2-hydroxy 2- (indol-3-ylmethyl) -4-keto glutaric acid (r-mp) and certain monatin stereoisomers, such as r, res, r monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the r, res, r, and salt configurations thereof. further, the present invention relates to a pair of primers, an expression cassette, a cloning vector or vehicle, recombinant peptides or polypeptides, a method for producing these peptides, a recombinant antibody, a method for generating a variant of a nucleic acid, a recombinant leader or signal sequence, methods for cleaving and forming a carbon-carbon bond, a composition, and methods for making a food, feed or beverage, as well as for converting a biomass or any lignocellulosic material in a fuel.esta presente invenção refere-se a polipeptídios com atividade de aldolase, incluindo atividade de piruvato tal como, sem limitação, atividade de hmg e/ou khg aldolase, polinucleotídeos codificando esses polipep- tídios, e métodos para produzir e usar esses polinucleotídeos e polipeptídios. em algumas modalidades, a presente invenção refere-se a polipeptídios com atividade
