Kemin Wang,Hui Shi,Xiaoxiao He,Xiaosheng Ye,Xu Wu,Qiuping Guo,Bing Zhou
申请号：
US13639843
公开号：
US20130251638A1
申请日:
2011.03.22
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The present invention relates to nucleic acid-based detection field, in particularly to a switchable nucleic acid aptamer probe and use thereof. The switchable nucleic acid aptamer probe includes a probe main body as well as a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment partially complemented with the nucleic acid aptamer fragment. The nucleic acid aptamer fragment and the nucleic acid fragment are linked by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and in vivo detection of tumor in living body. The probe of the invention holds relatively high stability, specificity and sensitivity, and its use is easy to operation, fast, sensitive, specific and of low cost.
