The present invention relates to a non-destructive process for the preparation of amoebocyte lysate from the haemolymph of horseshoe crab, characterized by the steps of firstly, means for acclimatizing a live specimen of horseshoe crab in treated seawater under controlled conditions then, means for cleaning the live specimen, including cleaning the base of the last pair of thoracic appendages of the live specimen next, means for withdrawing the haemolymph through the base of the last pair of thoracic appendage under aseptic condition then, means for transferring the collected haemolymph into a pre-cooled disposable centrifuge tube then, means for centrifuging the collected haemolymph to separate amoebocyte cells from lymph then, means for decanting the lymph out of the disposable centrifuge tube then, means for washing the white amoebocyte cells and centrifuging then, means for sonicating the amoebocyte cells for lysing of the amoebocyte cells then, means for storing the sonicated amoebocyte cells for further lysing of the amoebocyte cells then, means for centrifuging the sonicated mixture, thereby separating the amoebocyte lysate in the form of a supernatant then, means for decanting the amoebocyte lysate into a sterilized vessel then, means for pre-freezing the amoebocyte lysate and subsequently, freeze-drying the amoebocyte lysate.La présente invention concerne un procédé non destructif de préparation dun lysat damibocyte à partir de lhémolymphe du limule, caractérisé par les étapes consistant à utiliser tout dabord un moyen pour acclimater un spécimen vivant de limule dans de leau de mer traitée dans des conditions contrôlées puis un moyen pour nettoyer le spécimen vivant, cela comprenant le nettoyage de la base de la dernière paire dappendices thoraciques du spécimen vivant puis un moyen pour extraire lhémolymphe à travers la base de la dernière paire dappendices thoraciques dans des conditions aseptiques puis un moyen pour transférer lhémolymphe recueilli d
