Compositions and methods comprising high affinity monoclonal antibody conjugates against several DC receptors are described herein. The inventors prepared fusion proteins with antigens for DC-targeting vaccine generation by conjugating several M. tuberculosis protein antigens with high affinity monoclonal antibodies against several DC receptors with a view to developing novel human vaccines based on in vivo DC-targeting. The findings of studies described herein indicate that vaccines bearing TB antigens can recall a potent memory antigen-specific T cell response in vitro resulting in IFNγ secretion.本文中描述包含針對若干DC受體之高親和力單株抗體結合物(conjugates)之組合物及方法。本發明者藉由使若干結核分枝桿菌(M.tuberculosis)蛋白質抗原與針對若干DC受體之高親和力單株抗體結合來製備具有抗原的融合蛋白用於產生DC靶向疫苗,以開發基於活體內DC靶向之新穎人類疫苗。本文所述研究之結果表明帶有TB抗原之疫苗可在活體外喚起(recall)有效記憶抗原特異性T細胞反應,而引起IFNγ分泌。
