Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
发明人:
Butler, Andrew A.,Trevaskis, James L.
申请号：
AU2010202600
公开号：
AU2010202600B2
申请日:
2010.06.22
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
#$%^&*AU2010202600B220120719.pdf#####52 ABSTRACT The expression of a mRNA encoding a putative 76 amino acid, secreted protein ("Enhol") was found to negatively correlate with fasting triglyceride and cholesterol levels. A recombinant adenovirus was used to increase the expression of Enhol mRNA in two mouse models of obesity, KK-AY and Lepb"/Lepob mice. Over-expression of Enhol by adenovirus injection significantly, and reproducibly, reduced fasting triglyceride and cholesterol levels in both models. In addition, transgenic mice strains were made that over express Enhol protein. Additionally, the expression of a key gene involved in lipogenesis (fatty acid synthase) and FAS protein levels were reduced by ENHOI adenoviral treatment in Lep0 /Lepob mice. Full-length ENHOI peptide, or peptide derivatives, homologues, analogues, or mimetics thereof, delivered by oral intake, injection, subcutaneous patch, or intranasal routes, could be used as therapeutic or diagnostic agents for hypercholesterolemia, hypertriglyceridemia, insulin resistance, obesity, diabetes, and/or energy imbalance. 2313003_1 (GHMatters)1/24 FM (g) weight (g) -n FFM (g) % Increase in BW ncn % bodyfist 08 EmEoE I I
