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Method of isolating human cDNAs by transfecting a nucleic acid sequence of a non-plant donor into a host plant in an anti-sense orientation
专利权人:
KUMAGAI MONTO H.;DELLA-CIOPPA GUY R.;ERWIN ROBERT L.;MCGEE DAVID R.
发明人:
KUMAGAI MONTO H.,DELLA-CIOPPA GUY R.,ERWIN ROBERT L.,MCGEE DAVID R.
申请号:
US20020142077
公开号:
US2003077619(A1)
申请日:
2002.05.08
申请国别(地区):
美国
年份:
2003
代理人:
摘要:
The present invention provides a method of compiling a functional gene profile of an organism, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, a method of determining the presence of a trait in plant, and a method of isolating human cDNA. The methods comprise expressing transiently a nucleic acid sequence of a non-plant donor organism into a host plant by a viral vector to affect phenotypic or biochemical changes in the host plant. The present invention provides a method for discovering the presence of a new gene and determining its function and sequence in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host plant to knock out the endogenous gene expression.
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