A cultivation bag assembly for the cultivation of microbes, fungi and other organisms includes a bag having first and second walls joined along side edges to define a bag interior for containing a food substrate and an organism to be cultivated. At least one of the first and second walls is constructed of a layer of water-vapor permeable material to allow the passage of water vapor therethrough. A bag wall overlay layer formed from gas impermeable layer is releasably coupled to the bag. The overlay layer overlays the layer of water-vapor permeable material to prevent the passage of gases therethrough. The bag wall overlay layer has an opening with a gas filter patch covering the opening to allow the passage of oxygen and carbon dioxide gases through the gas filter patch to and from ambient air to facilitate incubation of the organism within the interior of the bag. The bag wall overlay layer is removable from the bag to allow water vapor to pass through the layer of water-vapor permeable material to ambient air facilitate drying of the bag contents after incubation is complete.本發明公開了一種用於培養微生物、真菌和其他生物體的培養袋組件，包括袋子，所述袋子具有第一和第二壁，所述第一和第二壁沿側邊連接以限定出用於容納食品底物和待培養生物體的袋子內部。所述第一和第二壁中的至少一者由水-蒸汽可透過材料層製成以允許水蒸氣通過其中。由氣體不可透過層形成的袋壁覆蓋層與袋子可拆卸偶接。所述覆蓋層覆蓋所述水-蒸汽可透過材料層以防止氣體通過其中。所述袋壁覆蓋層具有一開口，該開口上遮蓋有氣體過濾貼片以允許氧氣和二氧化碳氣體通過氣體過濾貼片排出到環境空氣或從環境空氣中進入袋子內部，以促進生物體在袋子內部培育。所述袋壁覆蓋層可從袋子中去除以允許水蒸氣通過水-蒸汽可透過材料層進入環境空氣中，以在培育完成後促進袋子內容物乾燥。
