SHANAFELT, DANIEL,DZIADOSZ, JASON,HANNA, JOHN,BERBUAM, MICHAEL,SHEAFFER, CHRISTINE A.
申请号：
ARP180100809
公开号：
AR111843A1
申请日:
2018.04.03
申请国别(地区):
AR
年份:
2019
代理人:
摘要：
1. This document is concerned with the production of hydrogenation and hydrogenation products, in particular with the improvement of the reproducibility, purity and stability of first and second generation hydrides, with purity of at least 95% in each region. High-pressure reverse liquid chromatography (RP-HPLC) measurements showed no neutral proteins. Claim 54: Production process of separated and pure superbinder I obtained or derived from C. Histolyticum, including (a) Fermentar C. Histolyticum； (b) harvesting of a fermentation process containing superbinder I and superbinder II；(c) Purification of No. 1 and No. 2 machines, including the following steps: first, through ion exchange filter to filter fermentation； second, using ammonium sulfate (1) to accelerate filter filter filter filter filter, which contains less than 80 ppm of zinc and less than 1.2 of zinc； PPM nickel； 3. Stop the precipitation products passing through aisle (c) (ii) again；Fourthly, the resuspension channel (c) (i) was placed in an aqueous column； (v) deoxyribonucleic acid (c) (iv) was separated from the filter by ion exchange chromatography (IEC)； and (v) the filter (c) (v) was separated. (d) Estimate the total amount of nitrogen dioxide in each part collected through (c) (vi)；(e) Estimate the amount of dioxygenase in each part collected through (c) (vi)；(f) A fraction containing hydrogen No. 1 separated from the peak value of nitrous oxide is synthesized into a component until the number of Polymerization No. 1 is approximately the same as the estimated amount of nitrogen dioxide in step (D).La presente se refiere a los campos de producción de colagenasa y de los productos de colagenasa, y en particular al mejoramiento de la reproductibilidad, la pureza y la estabilidad de las composiciones de colagenasa I y colagenasa II, en donde las composiciones son al menos 95% puras por área, medido por cromatografía líquida a alta presión de fase inversa (RP-HPLC) y están esencialmente libres de prote
