Liquid composition for microinsemination, unfertilized egg, fertilized egg and embryo utilization liquid composition, microfertilization method using them, and activation method of unfertilized egg, fertilized egg and embryo
PROBLEM TO BE SOLVED: To provide a liquid composition for microinsemination, an unfertilized egg, a fertilized egg and an embryo-utilizing liquid composition, which have few problems such as cytotoxicity, can reliably perform microinsemination, and improve pregnancy results. The microinsemination method used and the activation method of unfertilized eggs, fertilized eggs and embryos are provided. SOLUTION: 0-35 mmol / L sodium ion, 30-50 mmol / L potassium ion, 5-10 mmol / L magnesium ion, 0.5-2 mmol / L calcium ion, 15-35 mmol / L. A liquid composition for microfertilization containing L phosphate ion, 0 to 2 mmol / L chloride ion, 5 to 15 mmol / L carbonate ion, and 5 to 10 mmol / L sulfate ion, and pyruvate or its own. Unfertilized egg containing salt and lactic acid or a salt thereof, fertilized egg and embryo utilization liquid composition, microfertilization method using them, and activation method of unfertilized egg, fertilized egg and embryo. [Selection diagram] Fig. 1【課題】細胞毒性等の問題が少なく、確実に顕微授精を行うことが可能で、妊娠成績が向上する顕微授精用液体組成物、未受精卵、受精卵及び胚賦活用液体組成物、それらを用いた顕微授精方法並びに未受精卵、受精卵及び胚の賦活方法を提供する。【解決手段】０～３５ｍｍｏｌ／Ｌのナトリウムイオンと、３０～５０ｍｍｏｌ／Ｌのカリウムイオンと、５～１０ｍｍｏｌ／Ｌのマグネシウムイオンと、０．５～２ｍｍｏｌ／Ｌのカルシウムイオンと、１５～３５ｍｍｏｌ／Ｌのリン酸イオンと、０～２ｍｍｏｌ／Ｌの塩化物イオンと、５～１５ｍｍｏｌ／Ｌの炭酸イオンと、５～１０ｍｍｏｌ／Ｌの硫酸イオンを含む顕微授精用液体組成物、更にピルビン酸又はその塩及び乳酸又はその塩を含む未受精卵、受精卵及び胚賦活用液体組成物、それらを用いた顕微授精方法及び未受精卵、受精卵及び胚の賦活方法。【選択図】図１
