The invention relates to an amniotic fluid-derived preparation. The amniotic fluid-derived preparation leverages cellular and non-cellular constituent components of amniotic fluid for use across a broad range of therapeutic applications, including use by physicians and other healthcare providers in the surgical and minimally invasive medical therapy of a wide range of injuries and disease processes. The amniotic fluid-derived preparation concentrates available quantities of non-cellular bioactive proteins and cellular elements to enhance therapeutic efficacy in multiple clinical settings. The amniotic fluid-derived preparation may be intraoperatively transplanted at the recipient site using a needleless syringe, by non-operative percutaneous injection through a hypodermic needle, or by direct application.