Basically, this invention provides for an inbred corn line designated NPAF4543, methods for producing a corn plant by crossing plants of the inbred line NPAF4543 with plants of another corn plant. The invention relates to the various parts of inbred NPAF4543 including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line NPAF4543 and plants produced by said methods.