The present invention relates to a Capsicum annuum seed designated 35-172 RZ, which exhibits medium high resistance to powdery mildew, resistance to Tobamovirus pathotypes P0, P1, P1-2 and P1-2-3, and a medium maturation time. The present invention also relates to a Capsicum annuum plant produced by growing the 35-172 RZ seed. The invention further relates to methods for producing the pepper cultivar, represented by pepper variety 35-172 RZ.