Provided are a novel polyvalent human papillomavirus (HPV) DNA vaccine, a fusion protein used therein, and a polynucleotide encoding the fusion protein, and more specifically, a polyvalent HPV DNA vaccine composition including polynucleotides, which encode early protein antigen 6 (E6) of types 6, 11, 16, 18, 39, 45, and 56 HPV or immunogenic fragments thereof； and early protein antigen 7 (E7) of types 6, 11, 16, 18, 39, 45, and 56 human papillomavirus (HPV) or immunogenic fragments thereof, respectively.本發明提供一種新型的多價人類乳突病毒（HPV）DNA疫苗，裡面使用一種融合蛋白，以及一種編碼融合蛋白的聚核苷酸，還有更特別的是，一種包含分別可編碼6、11、16、18、39、45和56型人類乳突病毒（HPV）的早期蛋白抗原7(E7)或其免疫原性片段的聚核苷酸的多價HPV DNA疫苗成份。
