Kenneth R. CHIEN,Jonathan CLARKE,Miia LEHTINEN,Kylie FOO,Chuen Yan LEUNG
申请号：
US15805463
公开号：
US20180148691A1
申请日:
2017.11.07
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The present invention provides methods for isolating human cardiac ventricular progenitor cells (HVPs), wherein cultures of day 5-7 cardiac progenitor cells are negatively selected for one or more first markers expressed on human pluripotent stem cells, such as TRA-1-60, to thereby isolate HVPs. The methods can further include positive selection for expression of a second marker selected from the group consisting of JAG1, FZD4, LIFR, FGFR3 and TNFSF9. Large populations, including clonal populations, of isolated HVPs that are first marker negative/second marker positive are also provided. Methods of in vivo use of the HVPs for cardiac repair or to improve cardiac function are also provided. Methods of using the HVPs for cardiac toxicity screening of test compounds are also provided.
