PURPOSE: A manufacturing method of shampoos for depilation prevention and hair growth promotion is provided to form clean follicles and healthy hair roots without irritating skin and to minimize hair root damage caused by environmental factors by using environmentally friendly surfactants with high biodegradability. CONSTITUTION: A manufacturing method of shampoos for depilation prevention and hair growth promotion comprises the following steps: mixing a herbal medicine fermented product using cypress extract, biotae orientalis Folium extract, Acacia concinna, espinosilla extract, henna extract, smilax extract, and EM fermentation liquid with a native plant fermentation product, herbal medicine extract, natural betaine, sasa borealis extract, and alkali ion water(S1); melting polyquaternium and adding glucamate at 60 deg. Celsius to increase viscosity(S2); mixing with a surfactant(S3); and adding an additive which comprises a natural antiseptic and various herbal essential oils(S4). The surfactant comprises disodium laureth sulfosuccinate, LES, decyl glucoside, lauryl glucoside, sodium cocoamphoacetate, cocobetaine, soap glue, cocamide DEA, coconut fatty acid diethanolamide, ethylhexyl olivate, and sodium cocoyl apple amino acids. [Reference numerals] (S1) Step of mixing predetermined amounts of various plant extracts, herbal medicine fermented product, native plant fermented product, alkali ion water, and natural betaine; (S2) Step of increasing viscosity by adding polyquaterinium and glucamate; (S3) Step of mixing with a surfactant; (S4) Step of further adding natural antiseptic and various additives본 발명은 탈모방지 및 발모촉진용 샴푸 및 그 제조방법에 관한 것으로서, 더욱 상세하게는 한약재 성분과 자생식물을 포함하여 발모를 촉진함과 아울러 자연에 가까우면서 생분해도가 높은 친환경적인 계면활성제를 사용하여 환경적 요인에 의한 모근파괴를 최소화시킨 저자극성 천연 샴푸 및 그 제조방법을 제공함을 목적으로 한다.상기 목적을 달성하기 위해, 본 발명은 편백 추출물, 측백엽 추출물, 시카카이 추출물, 에스피노질리아 추출물, 헤나 추출물, 청미래 덩굴 추출물, EM 발효액을 사용하여 발효시킨 한약재 발효물과 자생식물 발효물, 한약재 우린물, 내츄럴 베타인, 조릿대 추출물 및 알칼리 이온수를 함유하고; 폴리쿼터 및 글루카메이트로 이루어진 점증제와, 디소듐라우레스