An apparatus of mechanical cell lysis where cells are subjected to high-pressure extrusion to bring biological samples such as microorganisms or animal cells or fragments of plant to bring into a dispersed/disintegrated state. The invention is an apparatus consisting of a single/multiple station piston-plunger arrangement made of any pressure, corrosion resistant material, the output of which is controlled by a novel relief valve which dispenses the contents automatically when the desired pressure is reached. The relief valve regulates the pressure depending upon the spring used. Spring of desired spring constant gives higher flexibility with high precision. The device with its novel relief valve provides more safety and clean and mild process for cell lysis.
